Journal: NPJ Precision Oncology

Article Title: IL-11/IL-11R signal inhibition by 9MW3811 remodels immune tumor microenvironment and enhances anti-tumor efficacy of PD-1 blockade

doi: 10.1038/s41698-025-00913-w

Figure Lengend Snippet: A IL-11 is more overexpressed in CHOL, ESCA, HNSC, LUSC, LUAD, COAD, CESC, and BRCA (data from The Cancer Genome Atlas (TCGA) than normal tissues (data from the Genotype-Tissue Expression (GTEx). B Kaplan–Meier survival analysis of IL-11 mRNA levels for BRCA, CESC, CHOL, ACC, LUAD, PAAD, UCS, and COAD with datasets from TCGA and GTEx. C Relative contributions of IL-11 in different cancer types. The scores were evaluated by AIBERT®. D The impact of genetic loss of IL-11Rα on tumor growth was evaluated using MC38 cell line-derived tumor models with IL-11Rα knockout (IL-11Rα KO -MC38). MC38 or IL-11Rα KO -MC38 cells were inoculated subcutaneously into separate groups of C57BL/6J mice. Data are presented as mean ± SEM, n = 8. E The effects of genetic loss of IL-11Rα on tumor growth were assessed in Hepa1-6 tumor models using C57BL/6J wild-type mice and IL-11Rα knockout mice ( IL-11Rα KO -mice ). Hepa1-6 cells were inoculated subcutaneously into separate groups of C57BL/6 J wild-type or IL-11Rα KO mice, Data are presented as mean ± SEM, n = 6.

Article Snippet: IL-11Rα/STAT3-luc HEK293 reporter cell line was constructed by transfecting the expression vector containing human IL-11Rα full-length gene (HG10252-UT, Sino Biological) into STAT3-Luc/HEK293 cells which constitutively express human gp130 STAT3-luciferase (CBPB0002, Nanjing Cobioer).

Techniques: Expressing, Derivative Assay, Knock-Out

Journal: NPJ Precision Oncology

Article Title: IL-11/IL-11R signal inhibition by 9MW3811 remodels immune tumor microenvironment and enhances anti-tumor efficacy of PD-1 blockade

doi: 10.1038/s41698-025-00913-w

Figure Lengend Snippet: A Binding activity of 9MW3811 with recombinant IL-11 from different species. B Binding activity of 9MW3811 with human IL-11, compared with X203, a reported anti-IL-11 neutralizing antibody. C Blocking activity of 9MW3811 against the formation of IL-11/IL-11Rα/gp130 protein complex, compared with X203. D Schematic representation of JAK/STAT3 signaling downstream of IL-11. E JAK/STAT3 signaling transduction inhibitions by 9MW3811 and X203 were performed by luciferase reporter gene assay, using IL-11Rα/STAT3-luc HEK293 cell line. F The inhibition of pSTAT3 stimulated by human IL-11 in HGC-27 tumor cells was detected by immunoblotting assay using Simple Western-Jess. Data is shown as mean± SD.

Article Snippet: IL-11Rα/STAT3-luc HEK293 reporter cell line was constructed by transfecting the expression vector containing human IL-11Rα full-length gene (HG10252-UT, Sino Biological) into STAT3-Luc/HEK293 cells which constitutively express human gp130 STAT3-luciferase (CBPB0002, Nanjing Cobioer).

Techniques: Binding Assay, Activity Assay, Recombinant, Blocking Assay, Transduction, Luciferase, Reporter Gene Assay, Inhibition, Western Blot, Simple Western

Journal: International Journal of Molecular Sciences

Article Title: Nonspecific Inhibition of IL6 Family Cytokine Signalling by Soluble gp130

doi: 10.3390/ijms25031363

Figure Lengend Snippet: sgp130Fc inhibits IL6, IL11, OSM, and CT1-induced STAT3 activation. ( A ) ELISA of IL6R, IL11RA, LIFR, and OSMR in the conditioned supernatant of A549, primary human hepatocytes (HEP), and primary human hepatic stellate cells (HSC) 17 h after plating. Data are shown as box-and-whisker plots with median (middle line), 25th–75th percentiles (box) and min-max percentiles (whiskers). Western blots of p -STAT3 and STAT3 in CNTF, CT1, IL6, IL11, LIF (5 ng/mL)-stimulated ( B ) A549, ( C ) HEP, ( D ) HSC (15 min) and ( B – D ) immunoblots of p -STAT3, STAT3, GAPDH in OSM (5 ng/mL)-stimulated A549, HEP, HSC (15 min) in the presence or absence of sgp130Fc (5 µg/mL). ( A – D ) n = 4 biological replicates. BL: baseline. n.d. not detected.

Article Snippet: The levels of IL6R, IL11RA, LIFR, and OSMR in an equal volume of cell culture supernatant were quantified using Human IL-6R alpha Quantikine ELISA kit (DR600, R&D Systems, Minneapolis, MN, USA), Human interleukin 11 receptor alpha (IL11RA) ELISA Kit (MBS453817, MyBioSource, San Diego, CA, USA), Human LIFR ELISA Kit (MBS2513036, MyBioSource, San Diego, CA, USA), or Human OSMR ELISA Kit (MBS2514091, MyBioSource, San Diego, CA, USA), respectively.

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay, Whisker Assay, Western Blot